Product Description

VCP Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91426-50, BS91426-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P55072(Human) Q01853(Mouse) P46462(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat, Zebrafish

Applications: WB, ICC/IF, IHC, FC, IP,

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Valosin containing protein (VCP), also designated TERA (for transitional endoplasmic reticulum ATPase) or p97, is a member of the AAA family of ATPases, which are involved in a variety of cellular activities. VCP is the mammalian homolog of Saccharomyces cerevisiae Cdc48, a protein essential for the completion of mitiosis in yeast. VCP is thought to be involved in a variety of membrane functions and in the regulation of the cell cycle. It associates with ubiquitinated IκB-α as well as with the 26S Proteosome, indicating a potential role for VCP in the proteosome-mediated degradation of IκB-α.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: VCP polyclonal antibody detects endogenous levels of VCP protein.

Bioworld Molecular Weight: 89 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of VCP on different cells lysates using anti-VCP antibody at 1/500 dilution.
Positive control:
Line 1: SH-SY5Y
Line 2: HepG2
Line 3: Hela, ICC staining VCP in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: TER ATPase, VCP, 15S Mg(2+) ATPase p97 subunit, 15S Mg(2+)-ATPase p97 subunit, ALS14, ATPase p97, CDC48, IBMPFD, MGC131997, MGC148092, MGC8560, p97, TER ATPase, TERA, TERA, Transitional endoplasmic reticulum ATPase, Valosin containing protein, Valosin-containing protein, VCP, Yeast Cdc48p homolog,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified