VDR Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91430-50, BS91430-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P11473(Human) P13053(Rat)
Host: Rabbit
Reactivity: Human, Rat
Applications: WB, ICC FC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
FC:1:50-1:100
Background: The active metabolite of vitamin D modulates the expression of a wide variety of genes in a developmentally specific manner. This secosteroid hormone can up- or downregulate the expression of genes involved in a diverse array of responses such as proliferation, differentiation and calcium homeostasis. 1,25-(OH)2-vitamin D3 exerts its effects through interaction with the vitamin D receptor (VDR), a member of the superfamily of hormone-activated nuclear receptors. In its ligand-bound state, the VDR forms heterodimers with the 9-cis retinoic acid receptor, RXR, and affects gene expression by binding specific DNA sequences known as hormone response elements, or HREs. In addition to regulating the above-mentioned cellular responses, 1,25-(OH)2-vitamin D3 exhibits antiproliferative properties in osteosarcoma, melanoma, colon carcinoma and breast carcinoma cells.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: VDR polyclonal antibody detects endogenous levels of VDR protein.
Bioworld Molecular Weight: 48 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Vitamin D Receptor on different cell lysate using anti-Vitamin D Receptor antibody at 1/2,000 dilution.
Positive control:
Lane 1: U937
Lane 2: SK-Br-3, ICC staining Vitamin D Receptor in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: VDR, NR1I1, 1 25 dihydroxyvitamin D3 receptor, 1, 1, 25 dihydroxyvitamin D3 receptor, 1, 25-@dihydroxyvitamin D3 receptor, 25-dihydroxyvitamin D3 receptor, Member 1, NR1I1, Nuclear receptor subfamily 1 group I member 1, PPP1R163, Protein phosphatase 1, regulatory subunit 163, VDR, VDR, Vitamin D (1, 25- dihydroxyvitamin D3) receptor, Vitamin D hormone receptor, Vitamin D nuclear receptor variant 1, Vitamin D receptor, Vitamin D3 receptor,
Immunogen: Recombinant protein with human Vitamin D Receptor aa 100-300.
Conjugate: Unconjugated
Modification: Unmodified