VE-Cadherin (phospho-Y731) Polyclonal Antibody-BS4242

VE-Cadherin (phospho-Y731) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4242-50, BS4242-100

Swiss-Prot: P33151

Host: Rabbit

VE-Cadherin (phospho-Y731) Polyclonal Antibody-BS4242

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: The cadherins are a family of Ca 2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy-terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including β-catenin, to regulate cadherin function. VE-cadherin (for vascular endothelial cadherin, also designated cadherin-5) is localized at intercellular junctions of endothelial cells, where it is thought to play a role in the cohesion and organization of intercellular junctions.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-VE-Cadherin (Y731) polyclonal antibody detects endogenous levels of VE-Cadherin protein only when phosphorylated at Tyr731

Bioworld Molecular Weight: ~ 130 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-VE-Cadherin (Y731) polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate treated with EGF (0.1ng/ml,30mins)
Lane 2: H9C2 cell lysate treated with EGF (0.1ng/ml,30mins), Immunohistochemistry (IHC) analyzes of p-VE-Cadherin (Y731) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Alternative Name: Cadherin-5; 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CD144; CDH5; Cadherin 5

Immunogen: Synthetic phosphopeptide derived from human VE-Cadherin around the phosphorylation site of Tyrosine 731.

Modification: Phosphorylation

Conjugate: Unconjugated