Product Description

VEGF-A (L98) Polyclonal Antibody

Catalogue Numbers: AP0742-50, AP0742-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P15692

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: VEGF-A (L98) polyclonal antibody detects endogenous levels of total VEGF-A protein (13 isoforms).

Extra Notes: Western blot (WB) analysis of VEGF-A (L98) pAb at 1:500 dilution
Lane1:LOVO whole cell lysate(40ug)
Lane2:Panc1 whole cell lysate(40ug)
Lane3:CT26 whole cell lysate(40ug)
Lane4:H9C2 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 27 kDa (VEGF206); 45 kDa (L-VEGF206);
~ 15-44 kDa (Others Isoform)

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF

Immunogen: Synthetic peptide, corresponding to amino acids 70-120 of Human VEGF206.

Conjugate: Unconjugated

Modification: Unmodified