VGLL2 Polyclonal Antibody
Catalogue Numbers: BS61467-50, BS61467-100
Sizes: 50µl, 100µl
Alternative Name: Transcription cofactor vestigial-like protein 2; Vgl-2; Protein VITO1; VGLL2; VITO1
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q8N8G2
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Vgl-2 (Transcription cofactor vestigial-like protein 2), also known as VITO-1, is a 317 amino acid protein that contains a domain through which it interacts with TEF-1, a protein that plays a role in controlling the expression of numerous genes. Specific to skeletal muscle, Vgl-2 is expressed highly in adult fast muscle and is expressed at lower levels in adult slow muscle and fetal skeletal muscle. During muscle differentiation, Vgl-2 mRNA levels increase and Vgl-2 translocates from the cytoplasm to the nucleus.Overexpression of Vgl-2 in MYOD-transfected 10T1/2 mouse embryonic fibroblasts increases expression of myosin heavy chain (MHC), which is a marker of terminal muscle differentiation. This evidence suggests that Vgl-2 is essential for muscle gene expression. There are two isoforms of Vgl-2 that are produced as a result of alternative splicing events.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: VGLL2 polyclonal antibody detects endogenous levels of VGLL2 protein.
Bioworld Molecular Weight: ~ 33 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of VGLL2 polyclonal antibody at 1:500 dilution
Lane 1: The Heart tissue lysate of Rat(40ug)
Lane 2: The Heart tissue lysate of Mouse(40ug)
Lane 3: Hela whole cell lysate(40ug)
Immunogen: Synthetic peptide, corresponding to amino acids 34-79 of Human VGLL2.
Conjugate: Unconjugated
Modification: Unmodified