Product Description

Villin Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66105-50, MB66105-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Villin is a member of the gelsolin family of calcium-regμlated actin-binding proteins. Unlike the ubiquitously expressed gelsolin, villin expression is restricted to simple epithelia of the gastrointestinal and urogenital tracts. It is localized to the apical cytoplasm and brush borders of these cells. Villin functions in the regμlation of actin dynamics in the apical epithelium, capping, nucleating and/or severing actin filaments in a calcium-dependent manner and regμlating cell shape in response to external stimμli. Phosphorylation of villin at Tyr60, 81, and 256 may be involved in the regμlation of cell migration. Expression of villin is increased in colorectal cancers, and villin-1 function appears to be involved in progressive cholestasis and hepatic failure.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IHC

Alternate Names: VIL, Villin-1

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Villin. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/100 - 1/300)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of Villin protein.

Molecular Weight: ~ 91 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P09327

Product: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Research Use Only