Product Description

villin1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91439-50, BS91439-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P09327(Human) Q62468(Mouse)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:2,000
ICC:1:500-1:2,000
IHC:1:50-1:200
FC:1:50-1:100

Background: Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular (CDh) and non-muscular (CDl) forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in non-muscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of Nebulin are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: villin1 polyclonal antibody detects endogenous levels of villin1 protein.

Bioworld Molecular Weight: 92/46 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Villin1 on different tissue lysates using anti-Villin1 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse colon
Lane 2: Human small intestine
Lane 3: Human colon
Lane 4: Rat kidney, ICC staining Villin1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: VIL1, VIL, D2S1471, OTTHUMP00000164145, VIL, VIL1, VILI, Villin 1, Villin-1, Villin1,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified