Product Description

Vinculin Polyclonal Antibody

Catalogue Numbers: BS67459-50, BS67459-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P18206

Host: Rabbit

Reactivity: Human, Mouse, Rat, Chicken, Pig

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development . Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail . In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration . Phospholipid binding to the tail domain and subsequent phosphorylation of vinculin at Ser1033 and Ser1045 by PKC-α and Tyr100 and Tyr1065 by Src kinases weakens the head-tail interaction . This change in vinculin allows the binding of a number of other proteins, including talin, α-actinin and paxillin, which disrupts the head-tail interaction and initiates the conformational change from the inactive to active state. Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth . This is supported by a demonstrated relationship between decreased vinculin expression and increased carcinogenesis and metastasis in colorectal carcinoma.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Vinculin protein.

Extra Notes: Western blot analysis of Vinculin expression in Hela (A), HepG2 (B), PC12 (C), mouse muscle (D) whole cell lysates.

Bioworld Molecular Weight: ~ 130 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Vinculin; Metavinculin; MV

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Vinculin. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified