Product Description

Vinculin Rabbit Monoclonal Antibody

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Catalogue Numbers: MB66403-50, MB66403-100

Sizes: 50µl, 100µl

Swiss-Prot: P18206

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of Vinculin protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Vinculin expression in K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates., Immunohistochemical analysis of Vinculin staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.18). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 124 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Vinculin; Metavinculin; MV

Immunogen: Recombinant protein of human Vinculin

Conjugate: Unconjugated

Modification: Unmodified