VIPR1 Polyclonal Antibody
Catalogue Numbers: BS60880-50, BS60880-100
Sizes: 50µl, 100µl
Alternative Name: Vasoactive intestinal polypeptide receptor 1; VIP-R-1; Pituitary adenylate cyclase-activating polypeptide type II receptor; PACAP type II receptor; PACAP-R-2; PACAP-R2; VPAC1; VIPR1
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P32241
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: The vasoactive intestinal peptide (VIP) and pituitary adenylate cylase-activating polypeptide (PACAP) belong to a superfamily of peptide hormones that include glucagon, secretin and growth hormone releasing hormone (1,2). The effects of VIP and PACAP are mediated by three G-protein coupled receptors, VPAC1, VPAC2 and the PACAP receptor (also designated PAC1-R) (1,2). The VPAC receptors have equal affinities for VIP and PACAP, which stimulate the activation of adenylyl cyclase (1,3). Both VPAC1 and VPAC2 are abundantly expressed in brain and T cells, where they modulate neuronal differentiation and T cell activation, respectively (1,4-6). The PACAP receptor is a seven transmembrane protein that produces at least eight isoforms by alternative splicing (2). Each isoform is associated with a specific signaling pathway and a specific expression pattern (2). The PACAP receptor, which is thought to play an integral role in brain development, preferentially binds PACAP in order to stimulate a cAMP-protein kinase A signaling pathway (2,7).
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: VIPR1 polyclonal antibody detects endogenous levels of VIPR1 protein.
Bioworld Molecular Weight: ~ 52 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of VIPR1 polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: H9C2 whole cell lysate
Lane 3: Raw264.7 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human VIPR1.
Conjugate: Unconjugated
Modification: Unmodified