Product Description

VNN1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: AP6008-50, AP6008-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O95497

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB IF

All Applications: WB: 1:500~1:2000
IF: 1:50~1:100

Background: Hematopoietic precursor cells migrate to the thymus, where they differentiate into mature T lymphocytes. GPI-anchored vanin-1 protein regulates the late adhesion steps of thymus homing of bone marrow precursor cells. Vanin-1 is ubiquitously expressed as a pantetheinase enzyme and catalyzes the hydrolysis of pantetheine for vitamin B5 recycling. The hydrolytic activity of vanin-1 generates the potent antioxidant cysteamine as a metabolite. As a membrane bound pantetheinase, vanin-1 provides the main source of cysteamine under normal physiological conditions. In mice, vanin-1 is expressed specifically in male Sertoli cells of the developing testis, where it aids in cell migration.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: VNN1 polyclonal antibody detects endogenous levels of VNN1 protein.

Bioworld Molecular Weight: ~ 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of extracts of various cell lines, using VNN1 antibody at 1:3000 dilution.

Alternative Name: Pantetheinase; Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1; Vanin-1; Vanin 1; Vanin1

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-415 of human VNN1 (NP_004657.2).

Conjugate: Unconjugated

Modification: Unmodified