Product Description

Von Willebrand Factor Polyclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: BS67414-50, BS67414-100

Sizes: 50µl, 100µl

Swiss-Prot: P04275

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: VWF (Von Willebrand factor) is a multimeric plasma glycoprotein that promotes adhesion of platelets to sites of vascular injury . Mature circulating VWF is made up of disulfide-bonded multimers that are in a complex with factor VIII . VWF is stored in secretory Weibel-Palade bodies in endothelial cells . It is synthesized as a large precursor protein and undergoes extensive posttranslational modifications including dimerization in the endoplasmic reticulum followed by cleavage of the pro-peptide and multimerization in the Golgi apparatus . VWF is important in hemostasis, and genetic defects in the structure and modification of VWF can cause von Willebrand disease (VWD), the most common congenital bleeding disorder in humans . Alternatively, increased levels of VWF have been shown to be involved in acute coronary thrombosis and are a clinical risk marker for atherosclerosis . VWF has also been shown to have a role in inflammation, functioning as an adhesive site for a variety of leukocyte subsets . Through siRNA experiments and the use VWF-deficient mice, it has also been shown that VWF regulates angiogenesis.

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of Von Willebrand Factor protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Von Willebrand Factor expression in mouse brain (A), rat brain (B), LOVO (C), HCT116 (D) whole cell lysates., Immunohistochemical analysis of Von Willebrand Factor staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 200 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: F8VWF; von Willebrand factor; vWF

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Von Willebrand Factor. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified