Product Description

VPAC1 (V371) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS3211-50, BS3211-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P32241

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: The vasoactive intestinal peptide (VIP) and the pituitary adenylate cylaseactivating polypeptide (PACAP) belong to a superfamily of peptide hormones that include glucagon, secretin and growth hormone releasing hormone. The effects of VIP and PACAP are mediated by three G-protein coupled receptors, VPAC1, VPAC2 and the PACAP receptor (also designated PAC1-R). The VPAC receptors have equal affinities for VIP and PACAP, which stimulate the activation of adenylyl cyclase. Both VPAC1, a 47 kDa protein, and VPAC2, a 65 kDa protein, are abundantly expressed in brain and T cells, where they modulate neuronal differentiation and T cell activation, respectively. The PACAP receptor is a seven transmembrane protein that produces at least eight isoforms by alternative splicing. Each isoform is associated with a specific signaling pathway and a specific expression pattern. The PACAP receptor, which is thought to play an integral role in brain development, preferentially binds PACAP in order to stimulate a cAMP-protein kinase A signaling pathway.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of VPAC1 (V371) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50. showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody,Secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 52 kDa

Specificity: VPAC1 (V371) polyclonal antibody detects endogenous levels of VPAC1 protein.

Alternative Name: VIPR1; VIPR1; PACAP-R2; VIP-R-1; Vasoactive intestinal polypeptide receptor 1

Immunogen: Synthetic peptide, corresponding to amino acids 350-400 of Human VPAC1.

Conjugate: Unconjugated

Modification: Unmodified