Product Description

VPS4B Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS61477-50, BS61477-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Class E vacuolar protein sorting (vps) proteins are necessary for appropriate sorting of receptors in the yeast endocytic pathway. The yeast Vps4p is a member of the AAA protein family (ATPases associated with diverse cellμlar activities) and plays an important role in transporting proteins out of a prevacuolar endosomal compartment. In human, two non-allelic orthologous proteins (VPS4A and VPS4B) of yeast Vps4p are known and can form heteromeric complexes with each other. Both VPS4 (also known as SKD1 in mouse) proteins are class E VPSs and are involved in intracellμlar protein trafficking, similar to Vps4p in yeast. A human CHMP1 protein, which is implicated in mμltivesicμlar body formation, physically interacts with VPS4 . HIV-1 uses cellμlar machinery to bud from infected cells and requires VPS4 and TSG101/VPS23 for this budding process. Dominant negative mutant of VPS4 inhibit vacuolar protein sorting and also arrest HIV-1 and MLV budding. Thus, retroviruses normally use the VPS pathway to form mμltivesicμlar bodies during the budding process.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Suppressor of K(+) transport growth defect 1, Protein SKD1, VPS4B, SKD1, VPS42, MIG1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: A synthetic peptide corresponding to residues in Human VPS4B.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: VPS4B polyclonal antibody detects endogenous levels of VPS4B protein.

Molecular Weight: ~ 49 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O75351

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only