VSNL1/VILIP-1 Polyclonal Antibody-BS8638

VSNL1/VILIP-1 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS8638-50, BS8638-100

Swiss-Prot: P62760

Host: Rabbit

VSNL1/VILIP-1 Polyclonal Antibody-BS8638

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 18KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of variousLysates, using VSNL1/VILIP-1 antibody at 1:430 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of U2OS cells using VSNL1/VILIP-1 antibody. Blue: DAPI for nuclear staining.

Alternative Name: VSNL1; HLP3; HPCAL3; HUVISL1; VILIP; VILIP-1

Immunogen: Recombinant fusion protein of human VSNL1/VILIP-1(NP_003376.2).

Modification: Unmodified

Conjugate: Unconjugated