Product Description

VWA5A Polyclonal Antibody

Catalogue Numbers: BS5627-50, BS5627-100

Sizes: 50µl, 100µl

Alternative Name: von Willebrand factor A domain-containing protein 5A; Breast cancer suppressor candidate 1; BCSC-1; Loss of heterozygosity 11 chromosomal region 2 gene A protein; BCSC1; LOH11CR2A

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O00534

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF

All Applications: WB: 1:500~1:1000
IF: 1:50~1:200

Background: VWA5A (von Willebrand factor A domain containing 5A), also known as BCSC-1 (breast cancer suppressor candidate 1) or LOH11CR2A (loss of heterozygosity 11 chromosomal region 2 gene A protein), is a 786 amino acid protein containing one VIT domain and one VWFA domain. VWA5A is expressed at low levels in various tissues, with no expression found in 80% of tumor cell lines. Likely acting as a tumor suppressor gene, deletion of VWA5A leads to loss of heterozygosity (LOH) in breast and ovarian tumors, and may have an important role as a potential gene therapy target. Abnormal expression of VWA5A may lead to an increase in adhesion of CNE-2L2 cells associated with an increase in expression of E-cadherin, alpha-catenin, and p53, resulting in a decrease of malignant activity in cells with ectopic expression of VWA5A.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: VWA5A polyclonal antibody detects endogenous levels of VWA5A protein.

Bioworld Molecular Weight: ~ 86 kDa (isoform1), 46 kDa (isoform3)

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of VWA5A polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: NIH-3T3 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 411-456 of Human VWA5A.

Conjugate: Unconjugated

Modification: Unmodified