Product Description

WIPI2 Polyclonal Antibody

Catalogue Numbers: BS67577-50, BS67577-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q9Y4P8

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents . It is generally activated by conditions of nutrient deprivation but is also associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer . The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes.
Vacuolar trafficking and autophagy are controlled by the class III type phosphoinositide 3-kinase (PI3K) Vps34, which generates phosphoinositide-3-phosphate (PtdIns3P) . Atg18 and Atg21 are two related WD-repeat proteins that bind PtdIns3P via a conserved Phe-Arg-Arg-Gly motif . It has been shown that Atg18 binds to Atg2 and that this complex is directed to vacuolar membranes by its interaction with PtdIns3P (8). Human orthologs of Atg18 and Atg21 were identified as members of the WD-repeat protein Interacting with Phosphoinositides (WIPI) family . WIPI1 (also called WIPI49) and WIPI2 have been shown to translocate from several vacuolar compartments to LC3-positive autophagosomes during autophagy; this translocation may be used as an autophagy marker .

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of WIPI2 protein.

Extra Notes: Western blot analysis of WIPI2 expression in HCT116 (A), mouse brain (B), rat brain (C) whole cell lysates.

Bioworld Molecular Weight: ~ 49 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: WD repeat domain phosphoinositide-interacting protein 2; WIPI-2; WIPI49-like protein 2

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human WIPI2. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified