Product Description

WNK1 (phospho-T60) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4208-50, BS4208-100

Swiss-Prot: Q9H4A3

Host: Rabbit

WNK1 (phospho-T60) Polyclonal Antibody-BS4208

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: WNK1 (WNK lysine deficient protein kinase 1), also known as KDP (kinase deficient protein), PSK, erythrocyte 65 kDa protein (p65), HSN2, HSAN2 or PRKWNK1, is a 2,382 amino acid cytoplasmic protein that participates in cell signaling, proliferation and survival, and regulates electrolyte homeostasis. WNK1 belongs to the Ser/Thr protein kinase family of the protein kinase superfamily, and contains one protein kinase domain. Existing as five alternatively spliced isoforms, WNK1 is widely expressed but is found at highest levels in skeletal muscke, heart, testis and kidney. The gene that encodes WNK1 maps to human chromosome 12p13.33, and when defective, is the cause of an an autosomal dominant disease known as pseudohypoaldosteronism type II (PHAII), as well as a hereditary sensory and autonomic neuropathy designated hereditary sensory and autonomic neuropathy type 2A (HSAN2A).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-WNK1 (T60) polyclonal antibody detects endogenous levels of WNK1 protein only when phosphorylated at Thr60.

Bioworld Molecular Weight: ~ 251 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-WNK1 (T60) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: HEK293T treated with EGF (100ng/ml,30 minutes) whole cell lysate (40ug)
Lane 3: PC12 whole cell lysate (40ug)
Lane 4: AML-12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-WNK1 (T60) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Alternative Name: Serine/threonine-protein kinase WNK1; Erythrocyte 65 kDa protein; Kinase deficient protein; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; hWNK1; WNK; HSN2; KDP; KIAA0344; PRKWNK1

Immunogen: Synthetic phosphopeptide derived from human WNK1 around the phosphorylation site of Threonine 60.

Modification: Phosphorylation

Conjugate: Unconjugated