Product Description

Wnt-1 (G331) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1777-50, BS1777-100

Sizes: 50µl, 100µl

Swiss-Prot: P04628

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: Products of the highly conserved Wnt gene family play key roles in regulating cellular growth and differentiation. The prototype member of the Wnt gene family, Wnt-1, is a cysteine-rich secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. β-catenin, a cadherin-binding cellular adhesion protein which also binds the tumor supressor gene APC, has been identified as a downstream target of a signal transduction pathway mediated by Wnt-1. Wnt-1 is essential for normal development of the embryonic nervous system and its expression is normally limited to the embryonic neural tube and adult spermatids. When improperly expressed in mammary tissue, Wnt-1 contributes to hyperplasia and tumorigenic progression. Wnt family members have been shown to interact with Sonic hedgehog (Shh) in vivo to induce myogenesis in somitic tissue.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Wnt-1 (G331) polyclonal antibody detects endogenous levels of Wnt-1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Wnt-1 (G331) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: Hela whole cell lysate (40ug)
Lane 3: DLD whole cell lysate (40ug)
Lane 4: The Testis tissue lysate of Mouse (40ug)
Lane 5: The Testis tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of Wnt-1 (G331) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 41 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Proto-oncogene Wnt-1; Proto-oncogene Int-1 homolog; WNT1; INT1; WNT 1

Immunogen: Synthetic peptide, corresponding to amino acids 301-350 of Human Wnt-1.

Conjugate: Unconjugated

Modification: Unmodified