WT1-AS Polyclonal Antibody-BS60050

WT1-AS Polyclonal Antibody

Catalogue Numbers: BS60050-50, BS60050-100

Sizes: 50µl, 100µl

Alternative Name: Putative Wilms tumor upstream neighbor 1 gene protein; WIT-1; Wilms tumor-associated antisense RNA; WT1-AS; WIT1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q06250

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Wilms’ tumor is a neoplasm of the kidneys that usually occurs in children and is characterized by the presence of abortive tubules and glomeruli surrounded by a spindled cell stroma. The 11p13 Wilms’ tumor locus consists of two coordinately regulated transcripts, WT1 and WIT-1, which are mutated in Wilms’ tumors. Wilms’ tumor upstream neighbor 1 (WIT-1) is encoded by an intron-less gene upstream of the Wilms’ tumor 1 (WT1) gene, which is is important for nephrogenesis and gonadal growth. The WT1 gene is bi-directionally transcribed from the same promoter region as WIT-1, which may function as an antisense regulator of WT1. WIT-1 and WT1 have the same temporal and cell-restricted expression pattern, although the expression of WIT-1 is less abundant. Methylation of the WIT-1 gene is implicated in hematologic malignancy of chemoresistant acute myeloid leukemia. Single nucleotide polymorphisms (SNPs) in the WIT-1 gene are significantly associated with focal segmental glomerulosclerosis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: WT1-AS polyclonal antibody detects endogenous levels of WT1-AS protein.

Bioworld Molecular Weight: ~ 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of WT1-AS polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human WT1-AS.

Conjugate: Unconjugated

Modification: Unmodified