Product Description

WT1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80004-50, BS80004-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P19544

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 49-60kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using WT1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Confocal immunofluorescence analysis of Hela cells using WT1 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining., Confocal immunofluorescence analysis of U-2 OS cells using WT1 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining., Immunofluorescence analysis of OVCAR-3 cells using WT1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: WT1; AWT1; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; WT33

Immunogen: Recombinant fusion protein of human WT1(P19544).

Conjugate: Unconjugated

Modification: Unmodified