WT1 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A1198-20, A1198-100
Citations, Manuals and MSDS Available upon request.
Background: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
Category: Polyclonal Antibodies
Applications: WB, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 49kDa
Observed Molecular Weight: 47-60kDa
Immunogen: Recombinant protein (or fragment)
Species: Human
GeneID: 7490
SWISS: P19544
Alternate Names: GUD; AWT1; WAGR; WT-1; WT33; NPHS4; WIT-2; WT1
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Epigenetics Nuclear Signaling, Transcription Factors, Cancer, Tumor biomarkers, Signal Transduction, ErbB-HER Signaling Pathway, Cell Biology Developmental Biology, Apoptosis.
NCBI Alias: WT1
Research Use Only