Product Description

XPB Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65890-50, BS65890-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P19447

Host: Rabbit

Reactivity: Human, Mouse, Rat,Chicken,Dog, Pig, Cow, Horse, Rabbit,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of 5 subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and MAT1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -63°C long term. Avoid freeze-thaw cycles.

Specificity: XPB Polyclonal Antibody detects endogenous levels of XPB protein.

Bioworld Molecular Weight: ~89kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes;Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (XPB) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C,

Alternative Name: Basic transcription factor 2 89 kDa subunit; BTF 2; BTF2; BTF2 p89; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; ERCC 3; ERCC3; ERCC3_HUMAN; Excision Repair Cross-complementing Rodent Repair deficiency complementation Group 3; GTF2H; RAD 25; RAD25; TFIIH 89 kDa subunit; TFIIH antibody TFIIH basal transcription factor complex 89 kDa subunit; TFIIH basal transcription factor complex helicase XPB subunit; TFIIH p89; Xeroderma pigmentosum group B-complementing protein.

Immunogen: KLH conjugated synthetic peptide derived from human XPB/ERCC3:601-700/782

Conjugate: Unconjugated

Modification: Unmodified