Product Description

XRCC5 (phospho-T714) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4240-50, BS4240-100

Swiss-Prot: P13010

Host: Rabbit

XRCC5 (phospho-T714) Polyclonal Antibody-BS4240

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: XRCC5 encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-XRCC5 (T714) polyclonal antibody detects endogenous levels of XRCC5 protein only when phosphorylated at Thr714

Bioworld Molecular Weight: ~ 82 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-XRCC5 (T714) pAb at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: A549 treated with UV for 5 minutes then repair for 1 hour whole cell lysate (40ug)
Lane 3: A549 treated with UV for 5 minutes then repair for 6 hours whole cell lysate (40ug)
Lane 4: The Brain tissue lysate of Mouse (40ug)
Lane 5: The Uterus tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of p-Ku-80 (T714) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Alternative Name: X-ray repair cross-complementing protein 5; 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku80; Ku86; Ku-80; Ku-86; Lupus Ku autoantigen protein p86; Nuclear factor IV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); XRCC5; G22P2

Immunogen: Synthetic phosphopeptide derived from human XRCC5 around the phosphorylation site of Threonine 714.

Modification: Phosphorylation

Conjugate: Unconjugated