XRCC5 (S462) Polyclonal Antibody-BS2692

XRCC5 (S462) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2692-50, BS2692-100

Sizes: 50µl, 100µl

Swiss-Prot: P13010

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: XRCC5 encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of v (D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo v (D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: XRCC5 (S462) polyclonal antibody detects endogenous levels of XRCC5 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of XRCC5 (S462) pAb at 1:500 dilution
Lane 1: PC3 whole cell lysate (40ug)
Lane 2: A549 whole cell lysate (20ug)
Lane 3: SK-OVCAR3 whole cell lysate (20ug)
Lane 4: The Testis tissue lysate of Mouse (40ug)
Lane 5: The Testis tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of Ku-86 (S462) pAb in paraffin-embedded human esophageal carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 82 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: X-ray repair cross-complementing protein 5; 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku80; Ku86; Ku-80; Ku-86; Lupus Ku autoantigen protein p86; Nuclear factor IV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); XRCC5; G22P2

Immunogen: Synthetic peptide, corresponding to amino acids 450-480 of Human XRCC5.

Conjugate: Unconjugated

Modification: Unmodified