XRCC6 (Acetyl-K338) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS64099-50, BS64099-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P12956
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: The Ku protein islocalized in the nucleus and iscomposed of subunits referred to as Ku-70 (or p70) and Ku-86 or (p86). Ku wasfirst described as an autoantigen to which antibodies were produced in a patient with scleroderma polymyositis overlap syndrome, and waslater found in the sera of patients with other rheumatic diseases. Both subunits of the Ku protein have been cloned, and a number of functions have been proposed for Ku, including cellsignaling, DNA replication and transcriptional activation. Ku is involved in Pol II-directed transcription byvirtue of its DNA binding activity,serving as the regulatory component of the DNA-associated protein kinase that phosphorylates Pol II and transcription factor Sp. Ku proteins also activate transcription from the U1 small nuclear RNA and the human transferrin receptor gene promoters. A Ku-related protein designated the enhancer binding factor (E1BF),composed of two subunits, has been identified as a positive regulator of RNA polymerase I transcription initiation.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: XRCC6 (Acetyl-K338) polyclonal antibody detects endogenous levels of XRCC6 protein only when acetylated at Lys338.
Bioworld Molecular Weight: ~ 69 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western Blot (WB) analysis of XRCC6 (Acetyl-K338) polyclonal antibody at 1:500 dilution
Lane1:Hela whole cell lysate(40ug)
Lane2:PC12 whole cell lysate(40ug))
Lane3:NIH-3T3 whole cell lysate(40ug)
Lane4:H9C2 whole cell lysate(40ug)
Lane5:HEK293T whole cell lysate(40ug)
Alternative Name: X-ray repair cross-complementing protein 6; 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; Lupus Ku autoantigen protein p70; Ku70; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 6; XRCC6
Immunogen: Synthetic Acetylated peptide derived from human XRCC6 around the Acetylation site of lysine K338.
Conjugate: Unconjugated
Modification: Acetylated