Product Description

YAF2 Polyclonal Antibody

Catalogue Numbers: BS60278-50, BS60278-100

Sizes: 50µl, 100µl

Alternative Name: YY1-associated factor 2; YAF2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8IY57

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Members of the myeloctyomatosis viral oncogene homologue (Myc) family, which include c-Myc, N-Myc and L-Myc, encode transcription factors that play a role in the acceleration of cell cycle progression and cell growth, inhibition of cell differentiation, and initiation of programmed cell death (apoptosis). YY1-associated factor 2, also known as YAF2, is a 180 amino acid protein that binds to c-Myc and inhibits c-Myc-mediated pathways. It also binds to N-Myc, enhancing N-Myc-dependent transcriptional activation. Furthermore, YAF2 interacts with YY1 to antagonize YY1 transactivation of muscle-specific promoters. Localized to the nucleus, YAF2 contains an N-terminal C2-X10-C2 zinc finger and exists as two isoforms.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: YAF2 polyclonal antibody detects endogenous levels of YAF2 protein.

Bioworld Molecular Weight: ~ 20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of YAF2 polyclonal antibody at 1:500 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human YAF2.

Conjugate: Unconjugated

Modification: Unmodified