Product Description

YB1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91460-50, BS91460-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P67809(Human) P62960(Mouse) P62961(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: Y-box binding protein YB-1 (also known as CCAAT-binding transcription factor, enhancer factor I subunit A and DNA-binding protein B) belongs to a family of multifunctional proteins which regulate both transcription and translation. Y-box proteins interact with a wide variety of nucleic acid structures to act as transcription factors and mRNA masking proteins. The modular structure of Y-box proteins includes a highly conserved N-terminal cold-shock domain (CSD, equivalent to the bacterial cold-shock proteins) and four basic C-terminal domains containing Arginine clusters and aromatic residues. YB-1 plays a role in cell proliferation as an activator of growth-associated gene expression. YB-1 is also a repressor of the cell death-associated gene FAS. YB-1 may play an important role in controlling cell survival by regulating the expression of cell growth-associated and death-associated genes.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: YB1 polyclonal antibody detects endogenous levels of YB1 protein.

Bioworld Molecular Weight: 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of YB1 on different lysates using anti-YB1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat, ICC staining YB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CBF-A, DBPB, EFI-A, YB-1, YBX1, BP 8, CBF-A, CCAAT binding transcription factor I subunit A, CCAAT-binding transcription factor I subunit A, CSDA2, CSDB, DBPB, DNA binding protein B, DNA-binding protein B, EFI-A, Enhancer factor I subunit A, MDR NF1, MGC104858, MGC110976, MGC117250, NSEP 1, NSEP1, Nuclease sensitive element binding protein 1, Nuclease-sensitive element-binding protein 1, p50, Q15905, Y-box binding protein 1, Y-box transcription factor, Y-box-binding protein 1, YB 1, YB-1, YBOX1, YBX 1, ybx1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified