Product Description

ZADH1 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS5987-50, BS5987-100

Sizes: 50µl, 100µl

Swiss-Prot: Q8N8N7

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC ICC

Background: ZADH1 (zinc binding alcohol dehydrogenase,domain containing 1), also known as PTGR2 (prostaglandin reductase 2), PGR2 or 15-oxoprostaglandin 13-reductase, is a 351 amino acid cytoplasmic protein that belongs to the NADP-dependent oxidoreductase L4BD family. Functioning as s a 15-oxoprostaglandin 13-reductase, ZADH1 catalyzes the conversion of 15-keto-prostaglandin E2 to 15-keto-13,14-dihydro-prostaglandin E2 in a NADPH-dependent manner. ZADH1 overexpression has been found to repress PPARγ transcriptional activity and adipocyte differentiation. Widely expressed, ZADH1 is found at highest levels in heart, kidney, liver, pancreas and prostate, with moderate levels found in brain, small intestine, lung, testis and skeletal muscle. ZADH1 exists as a monomer, utilizes NADPH as a cofactor, and undergoes alternative splicing to produce two isoforms that are encoded by a gene located on human chromosome 14q24.3.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ZADH1 polyclonal antibody detects endogenous levels of ZADH1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ZADH1 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate, Immunohistochemistry (IHC) analyzes of ZADH1 pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 38 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Prostaglandin reductase 2; PRG-2; PRG2; 15-oxoprostaglandin 13-reductase; Zinc-binding alcohol dehydrogenase domain-containing protein 1; PTGR2; ZADH1

Immunogen: Synthetic peptide, corresponding to amino acids 228-273 of Human ZADH1.

Conjugate: Unconjugated

Modification: Unmodified