Product Description

ZAK (Phospho-S165) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64561-50, BS64561-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NYL2

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ZAK (Phospho-S165) polyclonal antibody detects endogenous levels of ZAK protein only when phosphorylated at Ser165.

Bioworld Molecular Weight: ~ 91 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of ZAK (Phospho-S165) polyclonal antibody at 1:500 dilution
Lane A: SGC7901 whole cell lysate
Lane B: HEK293T whole cell lysate
Lane C: HCT116 whole cell lysate

Alternative Name: Mitogen-activated protein kinase kinase kinase MLT; Human cervical cancer suppressor gene 4 protein; HCCS-4; Leucine zipper- and sterile alpha motif-containing kinase; MLK-like mitogen-activated protein triple kinase; Mixed lineage kinase-related kinase; MLK-related kinase; MRK; Sterile alpha motif- and leucine zipper-containing kinase AZK; ZAK; MLTK; HCCS4

Immunogen: Synthetic phosphopeptide derived from human ZAK around the phosphorylation site of Serine 165.

Conjugate: Unconjugated

Modification: Phosphorylation