Product Description

ZBP-89 (E84) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2510-50, BS2510-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9UQR1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: ZBP-89, also known as BFCOL1, BERF1 and ZNF 148, is a zinc finger transcription factor that is universally expressed. ZBP-89, a Kruppel-like repressor protein, is the silencer element binding factor for Vimentin. ZBP-89 has been shown to bind to GC-rich DNA elements in promoters for gastrin, ornithine decarboxylase and the cyclin-dependent kinase inhibitor p21 (also designated Cip1 or WAF1). ZBP-89 expression is induced by trans-retinoic acid or butyrate, which also induces terminal differentiation of colon cancer cells. ZBP-89 cooperates with histone acetyltransferase coactivator p300 in the regulation of p21, a cyclin-dependent kinase inhibitor whose associated gene is a target gene of p53. ZBP-89 also regulates cell proliferation, in part, through its ability to directly bind the p53 protein and retard its nuclear export. Elevated levels of ZBP-89 induce growth arrest and apoptosis in human gastrointestinal cells.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ZBP-89 (E84) polyclonal antibody detects endogenous levels of ZBP-89 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ZBP-89 (E84) at 1:500 dilution
Lane 1: H9C2 whole cell lysate (40ug)
Lane 2: SP2/0 whole cell lysate (40ug)
Lane 3: Hela whole cell lysate (40ug)
Lane 4: HEK293T whole cell lysate (40ug)
Lane 5: U-87MG whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of ZBP-89 (E84) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 89 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Zinc finger protein 148; Transcription factor ZBP-89; Zinc finger DNA-binding protein 89; ZNF148; ZBP89

Immunogen: Synthetic peptide, corresponding to amino acids 53-98 of Human ZBP-89.

Conjugate: Unconjugated

Modification: Unmodified