Product Description

ZNF318 Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65684-50, BS65684-100

Swiss-Prot: Q5VUA4

Host: Rabbit

ZNF318 Polyclonal Antibody-BS65684

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat, Dog, Pig, Horse, Rabbit,

Applications: WB, IHC-P

All Applications: WB=1:500-2000 IHC-P=1:100-500

Background: ZNF318 is a 2279 amino acid endocrine regulatory protein that localizes to the nucleus. Highly expressed in testis, ovaries and kidneys, ZNF318 is a co-repressor of androgen receptor (AR)-mediated transcriptional activation and is thought to regulate transcription during spermatogenesis. ZNF318 interacts with the N-terminal domain of AR and contains two matrin-type zinc fingers. Two isoforms of ZNF318, designated TZF and TZF-L, are produced due to alternative splicing events. Each of these splice variants are thought to have unique roles in transcriptional regulation. While the TZF isoform functions as a repressor of AR-mediated transcriptional activation, the TZF-L isoform is thought to enhance AR-mediated transcriptional activation.

Purification and Purity: affinity purified by Protein A

Specificity: ZNF318 Polyclonal Antibody detects endogenous levels of ZNF318 protein.

Bioworld Molecular Weight: 125 kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Sample:
Lymph node (Mouse) Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
A549 (Human) Cell Lysate at 30 ug
HL60 (Human) Cell Lysate at 30 ug
293T (Human) Cell Lysate at 30 ug
Primary: Anti- ZNF318 at 1/1000 dilution, Paraformaldehyde-fixed, paraffin embedded (rat uterus)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF318) Polyclonal Antibody, Unconjugated (bs-11414R) at 1:200 overnight at 4°C

Alternative Name: Endocrine Regulatory Protein; HGNC13578; HRIHFB2436; ZFP318; Zinc Finger Protein 318; ZN318_HUMAN.

Immunogen: KLH conjugated synthetic peptide derived from human ZNF318

Modification: Unmodified

Conjugate: Unconjugated