Product Description

ZNF42 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS5810-50, BS5810-100

Sizes: 50µl, 100µl

Swiss-Prot: P28698

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC ICC

Background: Zinc finger genes that encode metal-binding proteins are transcriptional regulators of other genes. Myeloid zinc finger 1 (MZF-1), also designated Zinc finger protein 42, and transcription factor ZBP-89, also designated Zinc finger protein 148, belong to the Krueppel C2H2-type zinc-finger protein family. The gene encoding for the MZF-1 protein maps to chromosome 19q13.43 while the gene encoding for ZBP-89 is localized on chromosome 3q21. These proteins are nuclear proteins involved in the regulation of transcriptional events. MZF-1 regulates transcription during hemopoietic development and plays a role in myeloid cell differentiation. MZF-1 regulates the CD34 promoter in a tissue-specific manner. MZF-1 and FHL3 can form a complex of high molecular mass with other proteins in the nucleus. It is induced by retinoic acid and is primarily expressed in differentiating myeloid cells.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ZNF42 polyclonal antibody detects endogenous levels of ZNF42 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ZNF42 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lyate, Immunohistochemistry (IHC) analyzes of ZNF42 pAb in paraffin-embedded human stomach carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 56, 82 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Myeloid zinc finger 1; MZF-1; Zinc finger and SCAN domain-containing protein 6; Zinc finger protein 42; MZF1; MZF; ZNF42; ZSCAN6

Immunogen: Synthetic peptide, corresponding to amino acids 1-46 of Human ZNF42.

Conjugate: Unconjugated

Modification: Unmodified