Product Description

ZNF50 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS5985-50, BS5985-100

Sizes: 50µl, 100µl

Swiss-Prot: P10073

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krueppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZSCAN22 (zinc finger and SCAN domain-containing protein 22), also known as HKR2 or ZNF50 (zinc finger protein 50), is a 491 amino acid nuclear protein. Belonging to the Krueppel C2 H2-type zinc-finger protein family, ZSCAN22 contains eight C2 H2-type zinc fingers and one SCAN box domain. ZSCAN22 may be involved in transcriptional regulation, and becomes phosphorylated upon DNA damage.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ZNF50 polyclonal antibody detects endogenous levels of ZNF50 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ZNF50 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate, Immunohistochemistry (IHC) analyzes of ZNF50 pAb in paraffin-embedded human stomach carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 55 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Zinc finger and SCAN domain-containing protein 22; Krueppel-related zinc finger protein 2; Protein HKR2; Zinc finger protein 50; ZSCAN22; HKR2

Immunogen: Synthetic peptide, corresponding to amino acids 363-408 of Human ZNF50.

Conjugate: Unconjugated

Modification: Unmodified