Product Description

ZNRF2 Polyclonal Antibody

Catalogue Numbers: BS61455-50, BS61455-100

Sizes: 50µl, 100µl

Alternative Name: E3 ubiquitin-protein ligase ZNRF2; Protein Ells2; RING finger protein 202; Zinc/RING finger protein 2; ZNRF2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8NHG8

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). ZNRF2 (zinc and ring finger 2), also known as RNF202, is a 242 amino acid peripheral membrane protein that contains one RING-type zinc finger and localizes to the lysosome, as well as the endosome and the cell junction. Expressed at high levels in brain tissue, ZNRF2 is thought to function as an E3 ubiquitin-protein ligase that may be involved in the establishment and maintenance of neuronal transmission and plasticity. Upon DNA damage, ZNRF2 is subject to phosphorylation, probably by ATR or ATM.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ZNRF2 polyclonal antibody detects endogenous levels of ZNRF2 protein.

Bioworld Molecular Weight: ~ 24 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of TRAPPC6A polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate(40ug)
Lane 2: H9C2 whole cell lysate(40ug)
Lane 3: RAW264.7 whole cell lysate(40ug)
Lane 4: A549 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 155-200 of Human ZNRF2.

Conjugate: Unconjugated

Modification: Unmodified