Product Description

ZO-1 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71522-50, BS71522-100

Swiss-Prot: Q07157

Host: Rabbit

ZO-1 Polyclonal Antibody-BS71522

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 250KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of HeLa, using ZO-1 antibody at 1:2250 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of MCF7 cells using ZO-1 Rabbit pAb at dilution of 1:150. Blue: DAPI for nuclear staining.

Alternative Name: TJP1; ZO-1

Immunogen: Recombinant fusion protein of human TJP1(NP_003248.3).

Modification: Unmodified

Conjugate: Unconjugated