Product Description

ZO1 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9802M-50, BS9802M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q07157

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: ICC IHC

All Applications: ICC: 1:200
IHC: 1:200

Background: Tight junction protein ZO-1 is a protein that in humans is encoded by the TJP1 gene. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. ZO1 plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of ZO1 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 194 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZO1 antibody. Counter stained with hematoxylin., ICC staining ZO1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Tight junction protein ZO-1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1; TJP1; ZO1

Immunogen: This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to ZO1.

Conjugate: Unconjugated

Modification: Unmodified