ZP3 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A13156-20, A13156-100
Citations, Manuals and MSDS Available upon request.
Background: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene.
Category: Polyclonal Antibodies
Applications: WB, IF-P, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 47kDa
Observed Molecular Weight: 47kDa
Immunogen: Synthetic peptide
Species: Human
GeneID: 7784
SWISS: P21754
Alternate Names: ZPC; ZP3A; ZP3B; Zp-3; OOMD3; OZEMA3; ZP3
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:2000 IF-P, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
NCBI Alias: ZP3
Research Use Only