Product Description

ZYX Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-67414-60, E-AB-67414-120, E-AB-67414-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: ZYX

Target Synonym: ZYX; ESP-2; HED-2; zyxin

Research Areas: Epigenetics and Nuclear Signaling, Neuroscience, Signal Transduction

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB, IHC, IF

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q15942

Background: Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human ZYX (NP_001010972.1).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200

Calculated MW: 45kDa/61kDa

Observed MW: 78kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only