Product Description

Zyxin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91467-50, BS91467-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15942(Human) Q62523(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC/IF, IHC, FC, IP,

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Zyxin is a low abundance phosphoprotein localized to focal adhesion plaques and is thought to perform regulatory functions at these regions. The protein contains a number of proline-rich sequences as well as three LIM domains, zinc finger domains involved in protein binding. Zyxin interacts with several other proteins at cell adhesion sites, including members of the CRP (cysteine-rich protein) LIM domain containing protein family. The proline-rich domain of Zyxin associates with an SH3 domain of p95 Vav, but not with similar SH3 domains containing proteins such as GRB2 or PLCg. Zyxin has also been shown to interact with the focal adhesion protein VASP and may assist in the targeting of VASP to focal adhesions, microfilaments and membrane regions of high dynamic activity.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Zyxin polyclonal antibody detects endogenous levels of Zyxin protein.

Bioworld Molecular Weight: 75/61/45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Zyxin on MCF-7 cell using anti-Zyxin antibody at 1/500 dilution., ICC staining Zyxin in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: ZYX, ESP 2, ESP2, HED 2, HED2, ZYX, ZYX protein, Zyxin 2, Zyxin2,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified