Imprint U-shaped low-attachment wells in agarose for 3D cell aggregation
Size: 1 unit (GRE-MOU-U)
Stampwell U provides a reproducible, cheap & fast method for 3D cell culture. These 3D-printed stamps are designed to easily create U-bottom wells in agarose. Simply load your cell suspension and watch cells aggregate into arrays of individual 3D cell structures. Turn any petri dish into a low-adherent multiwell plate in no time!
Fast: mold your wells in less than an hour and get 81x 3D cell aggregates from a single cell suspension
Reusable: no more throwing away hundreds of expensive low-attachment plates. With Stampwell, you can create your own U-bottom wells whenever you want and repeat as many times as you like
Cost-saving: Stampwell is reusable: use one stamp for infinite quantities of U-bottom micro-wells
Simple: no need for sophisticated equipment or expertise, only standard agarose and a petri dish. With Stampwell, 3D cell culture is accessible to anyone !
How does Stampwell U work ?
Kit content of Stampwell U
1x Stampwell to mold an array of 81x U-bottom microwells.
Applications of Stampwell U
Compatible assays:
- Cell aggregation (spheroids & organoids)
- 3D cell culture
Once formed, spheroids or organoids can be assayed in situ (i.e. immunostaining, growth assays) or recovered for downstream assays (individual picking or bulk analysis). For optimal imaging of 3D structures, transfer them into our dedicated Stampwell V-shape.
Plate compatibility:
The stamp has a total diameter of 26 mm. It can be used in:
- 35 mm circular petri dishes with an aperture of at least 22 mm
- any larger vessel (i.e. 6-well plates)
Cell types:
So far, Stampwell has been successfully used to make multiple spheroids with HEK, HeLa, U-87 & U2-OS cell lines.
Results
Multiple spheroid formation in Stampwell U-shape microwells
U-bottom microwells were molded in agarose 2% using Stampwell. HeLa (left), HEK-293T (middle) or U-87 cells (right) were loaded at 850, 2800 or 1000 cells per well respectively and cultured in DMEM + 10% FBS. Phase-contrast pictures were taken after 2 (D2) or 3 (D3) days as indicated.
Image credits (HEK-293T cells): Laëtitia Landrique - Plateforme VoxCell, UAR TBMcore CNRS 3427, INSERM US05, Université de Bordeaux - 2023
Influence of seeding density on cell aggregate size, cultured in wells molded with Stampwell U-shape
Credits: Gaëlle Recher & Camille Douillet - CNRS, 2022 >
Additional resources
> Stampwell U-shape - Datasheet> Stampwell U-shape - Certificate of Analysis