β-TrCP/BTRC Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A1656-20, A1656-100
Citations, Manuals and MSDS Available upon request.
Background: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6.
Category: Polyclonal Antibodies
Applications: WB, IHC-P, IF/ICC, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 69kDa
Observed Molecular Weight: 65kDa
Immunogen: Recombinant protein (or fragment)
Species: Human
GeneID: 8945
SWISS: Q9Y297
Alternate Names: FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP; β-TrCP/BTRC
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 IHC-P, 1:50 - 1:200 IF/ICC, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Signal Transduction, Cell Biology Developmental Biology, Cell Cycle, Centrosome, Ubiquitin, Ubiquitin-Proteasome Signaling Pathway, Wnt -Catenin Signaling Pathway, Immunology Inflammation, NF-kB Signaling Pathway, Neuroscience.
NCBI Alias: BTRC
Research Use Only