CTCF Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A1133-20, A1133-100
Citations, Manuals and MSDS Available upon request.
Background: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT) -containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC) -containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Category: Polyclonal Antibodies
Applications: WB, IP, IF/ICC, IHC-P, ChIP, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 83kDa
Observed Molecular Weight: 130 kDa
Immunogen: Recombinant protein (or fragment)
Species: Human
GeneID: 10664
SWISS: P49711
Alternate Names: MRD21; FAP108; CFAP108; CTCF
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:2000 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells IF/ICC, 1:50 - 1:200 IHC-P, 1:50 - 1:200 ChIP, 5μg antibody for 10μg-15μg of Chromatin ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Epigenetics Nuclear Signaling.
NCBI Alias: CTCF
Research Use Only