DNA Ligase IV Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A1743-20, A1743-100
Citations, Manuals and MSDS Available upon request.
Background: The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V (D) J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
Category: Polyclonal Antibodies
Applications: WB, IHC-P, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 104kDa
Observed Molecular Weight: 110kDa
Immunogen: Recombinant protein (or fragment)
Species: Human
GeneID: 3981
SWISS: P49917
Alternate Names: LIG4S; DNA Ligase IV
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 IHC-P, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Epigenetics Nuclear Signaling, Cell Biology Developmental Biology, Apoptosis.
NCBI Alias: LIG4
Research Use Only