F10 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A1452-20, A1452-100
Citations, Manuals and MSDS Available upon request.
Background: This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.
Category: Polyclonal Antibodies
Applications: WB, IF/ICC, ELISA
Cross-reactivity: Human, Mouse
Protein Weight: 55kDa
Observed Molecular Weight: 75kDa
Immunogen: Recombinant protein (or fragment)
Species: Human
GeneID: 2159
SWISS: P00742
Alternate Names: FX; FXA; F10
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:2000 IF/ICC, 1:10 - 1:100 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Cardiovascular, Blood.
NCBI Alias: F10
Research Use Only