Fluorescein Isothiocyanate/FITC Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A20385-20, A20385-100
Citations, Manuals and MSDS Available upon request.
Background: Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is also widely used as a hapten to label different proteins.
Category: Polyclonal Antibodies
Applications: WB, ELISA
Cross-reactivity: Species independent
Observed Molecular Weight: 25kDa/55kDa
Immunogen: Chemical compounds corresponding to Fluorescein Isothiocyanate/FITC.
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:2000 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
NCBI Alias: FITC
Research Use Only