GLUD1 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A7631-20, A7631-100
Citations, Manuals and MSDS Available upon request.
Background: This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X.
Category: Polyclonal Antibodies
Applications: WB, IHC-P, IF/ICC, IP, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 61kDa
Observed Molecular Weight: 52kDa
Immunogen: Recombinant protein (or fragment) .
Species: Human
GeneID: 2746
SWISS: P00367
Alternate Names: GDH; GDH1; GLUD; hGDH1; GLUD1
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 IHC-P, 1:50 - 1:200 IF/ICC, 1:50 - 1:200 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Cancer, Signal Transduction, Cell Biology Developmental Biology, Endocrine Metabolism, Amino acid metabolism.
NCBI Alias: GLUD1
Research Use Only