HCoV-229E Spike S2 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A20394-20, A20394-100
Citations, Manuals and MSDS Available upon request.
Background: S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Category: Polyclonal Antibodies
Applications: WB, ELISA
Cross-reactivity: HCoV-229E
Protein Weight: 129kDa
Observed Molecular Weight: 160kDa
Immunogen: Synthetic peptide
GeneID: 918758
SWISS: P15423
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
NCBI Alias: HCoV-229E Spike S2
Research Use Only