IDH2 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A7190-20, A7190-100
Citations, Manuals and MSDS Available upon request.
Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+) . Five isocitrate dehydrogenases have been reported: three NAD (+) -dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+) -dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP (+) -dependent isozyme is a homodimer. The protein encoded by this gene is the NADP (+) -dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.
Category: Polyclonal Antibodies
Applications: WB, IHC-P, IF/ICC, IP, ELISA, ChIP
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 51kDa
Observed Molecular Weight: 43kDa
Immunogen: Recombinant protein (or fragment) .
Species: Human
GeneID: 3418
SWISS: P48735
Alternate Names: IDH; IDP; IDHM; IDPM; ICD-M; IDH-2; D2HGA2; mNADP-IDH; IDH2
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 IHC-P, 1:50 - 1:200 IF/ICC, 1:20 - 1:50 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP, 5μg antibody for 10μg-15μg of Chromatin
Research Areas: Epigenetics Nuclear Signaling, Cancer, Signal Transduction, Endocrine Metabolism, Mitochondrial metabolism, Mitochondrial markers.
NCBI Alias: IDH2
Research Use Only