[KO Validated] ANAPC5 Rabbit pAb - A7109

[KO Validated] ANAPC5 Rabbit pAb

Sizes: 20μL, 100μL

Catalogue Numbers: A7109-20, A7109-100

Citations, Manuals and MSDS Available upon request.

Background: This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.

Category: Polyclonal Antibodies

Applications: WB, IHC-P, IP, ELISA

Cross-reactivity: Human, Mouse, Rat

Protein Weight: 85kDa

Observed Molecular Weight: 85kDa

Immunogen: Recombinant protein (or fragment) .

Species: Human

GeneID: 51433

SWISS: Q9UJX4

Alternate Names: APC5; C5

Source: Rabbit

Isotype: IgG

Purity: Affinity purification

Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.

Recommended Dilutions: WB, 1:500 - 1:1000 IHC-P, 1:50 - 1:200 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Research Areas: Cell Biology Developmental Biology, Cell Cycle, Ubiquitin, Ubiquitin-Proteasome Signaling Pathway.

NCBI Alias: ANAPC5

Research Use Only